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primary rabbit antizo 1 antibody  (Proteintech)


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    Structured Review

    Proteintech primary rabbit antizo 1 antibody
    Primary Rabbit Antizo 1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 2532 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+antizo+1/ZO-1+Antibody/pm41204333-102-33-38
    Average 97 stars, based on 2532 article reviews
    primary rabbit antizo 1 antibody - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Lysis:

    Article Title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse.
    Article Snippet: .. Tissue powders or cells were extracted and homogenized with the lysis buffer containing 0.2% SDS (cells, lung, liver, brain, intestine, kidney) or 1% SDS (cochlea), 50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM ethylene diamine tetra-acetic acid (EDTA) pH 8.0, 1% Triton X-100 (A110694-0500, Sangon Biotech) and 1 mM phenylmethylsulfonyl fluoride (PMSF) (ST005, Beyotime) containing the cOmplete Protease Inhibitor Cocktail (11697498001, Roche) at 4°C for 10 min. Proteins were subjected to SDS–PAGE and transfer to PVDF membrane and analyzed by following antibodies: rabbit anti-Cingulin (1:1,000, 21369-1-AP, Proteintech), mouse anti-Cingulin (1:1,000, sc-365264, Santa Cruz), mouse anti-Flag (1:2,000, 30503ES60, Yeasen), rabbit anti-EGFP (1:2,000, 31002ES60, Yeasen), rabbit antiZO-1 (1:1,000, 21773-1-AP, Proteintech), rabbit anti-Occludin (1:1,000, 13409-1-AP, Proteintech), mouse anti-b-actin (1:20,000, 4970, Cell Signaling Technology) antibodies followed by incubation in HRP conjugated anti-Rabbit (1:5,000, BS13278, Bioworld) or antimouse (1:5,000, BS12478, Bioworld) secondary antibody for 2 h at room temperature (RT). .. The signals were visualized using ECL substrate (180-5001, Tanon) on an automatic chemiluminescence/fluorescence image analysis system (Tanon 4600, Tanon).

    Article Title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse
    Article Snippet: .. Tissue powders or cells were extracted and homogenized with the lysis buffer containing 0.2% SDS (cells, lung, liver, brain, intestine, kidney) or 1% SDS (cochlea), 50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM ethylene diamine tetra-acetic acid (EDTA) pH 8.0, 1% Triton X-100 (A110694-0500, Sangon Biotech) and 1 mM phenylmethylsulfonyl fluoride (PMSF) (ST005, Beyotime) containing the cOmplete Protease Inhibitor Cocktail (11697498001, Roche) at 4°C for 10 min. Proteins were subjected to SDS–PAGE and transfer to PVDF membrane and analyzed by following antibodies: rabbit anti-Cingulin (1:1,000, 21369-1-AP, Proteintech), mouse anti-Cingulin (1:1,000, sc-365264, Santa Cruz), mouse anti-Flag (1:2,000, 30503ES60, Yeasen), rabbit anti-EGFP (1:2,000, 31002ES60, Yeasen), rabbit antiZO-1 (1:1,000, 21773-1-AP, Proteintech), rabbit anti-Occludin (1:1,000, 13409-1-AP, Proteintech), mouse anti-b-actin (1:20,000, 4970, Cell Signaling Technology) antibodies followed by incubation in HRP conjugated anti-Rabbit (1:5,000, BS13278, Bioworld) or antimouse (1:5,000, BS12478, Bioworld) secondary antibody for 2 h at room temperature (RT). .. The signals were visualized using ECL substrate (180-5001, Tanon) on an automatic chemiluminescence/fluorescence image analysis system (Tanon 4600, Tanon).

    Protease Inhibitor:

    Article Title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse.
    Article Snippet: .. Tissue powders or cells were extracted and homogenized with the lysis buffer containing 0.2% SDS (cells, lung, liver, brain, intestine, kidney) or 1% SDS (cochlea), 50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM ethylene diamine tetra-acetic acid (EDTA) pH 8.0, 1% Triton X-100 (A110694-0500, Sangon Biotech) and 1 mM phenylmethylsulfonyl fluoride (PMSF) (ST005, Beyotime) containing the cOmplete Protease Inhibitor Cocktail (11697498001, Roche) at 4°C for 10 min. Proteins were subjected to SDS–PAGE and transfer to PVDF membrane and analyzed by following antibodies: rabbit anti-Cingulin (1:1,000, 21369-1-AP, Proteintech), mouse anti-Cingulin (1:1,000, sc-365264, Santa Cruz), mouse anti-Flag (1:2,000, 30503ES60, Yeasen), rabbit anti-EGFP (1:2,000, 31002ES60, Yeasen), rabbit antiZO-1 (1:1,000, 21773-1-AP, Proteintech), rabbit anti-Occludin (1:1,000, 13409-1-AP, Proteintech), mouse anti-b-actin (1:20,000, 4970, Cell Signaling Technology) antibodies followed by incubation in HRP conjugated anti-Rabbit (1:5,000, BS13278, Bioworld) or antimouse (1:5,000, BS12478, Bioworld) secondary antibody for 2 h at room temperature (RT). .. The signals were visualized using ECL substrate (180-5001, Tanon) on an automatic chemiluminescence/fluorescence image analysis system (Tanon 4600, Tanon).

    Article Title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse
    Article Snippet: .. Tissue powders or cells were extracted and homogenized with the lysis buffer containing 0.2% SDS (cells, lung, liver, brain, intestine, kidney) or 1% SDS (cochlea), 50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM ethylene diamine tetra-acetic acid (EDTA) pH 8.0, 1% Triton X-100 (A110694-0500, Sangon Biotech) and 1 mM phenylmethylsulfonyl fluoride (PMSF) (ST005, Beyotime) containing the cOmplete Protease Inhibitor Cocktail (11697498001, Roche) at 4°C for 10 min. Proteins were subjected to SDS–PAGE and transfer to PVDF membrane and analyzed by following antibodies: rabbit anti-Cingulin (1:1,000, 21369-1-AP, Proteintech), mouse anti-Cingulin (1:1,000, sc-365264, Santa Cruz), mouse anti-Flag (1:2,000, 30503ES60, Yeasen), rabbit anti-EGFP (1:2,000, 31002ES60, Yeasen), rabbit antiZO-1 (1:1,000, 21773-1-AP, Proteintech), rabbit anti-Occludin (1:1,000, 13409-1-AP, Proteintech), mouse anti-b-actin (1:20,000, 4970, Cell Signaling Technology) antibodies followed by incubation in HRP conjugated anti-Rabbit (1:5,000, BS13278, Bioworld) or antimouse (1:5,000, BS12478, Bioworld) secondary antibody for 2 h at room temperature (RT). .. The signals were visualized using ECL substrate (180-5001, Tanon) on an automatic chemiluminescence/fluorescence image analysis system (Tanon 4600, Tanon).

    Membrane:

    Article Title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse.
    Article Snippet: .. Tissue powders or cells were extracted and homogenized with the lysis buffer containing 0.2% SDS (cells, lung, liver, brain, intestine, kidney) or 1% SDS (cochlea), 50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM ethylene diamine tetra-acetic acid (EDTA) pH 8.0, 1% Triton X-100 (A110694-0500, Sangon Biotech) and 1 mM phenylmethylsulfonyl fluoride (PMSF) (ST005, Beyotime) containing the cOmplete Protease Inhibitor Cocktail (11697498001, Roche) at 4°C for 10 min. Proteins were subjected to SDS–PAGE and transfer to PVDF membrane and analyzed by following antibodies: rabbit anti-Cingulin (1:1,000, 21369-1-AP, Proteintech), mouse anti-Cingulin (1:1,000, sc-365264, Santa Cruz), mouse anti-Flag (1:2,000, 30503ES60, Yeasen), rabbit anti-EGFP (1:2,000, 31002ES60, Yeasen), rabbit antiZO-1 (1:1,000, 21773-1-AP, Proteintech), rabbit anti-Occludin (1:1,000, 13409-1-AP, Proteintech), mouse anti-b-actin (1:20,000, 4970, Cell Signaling Technology) antibodies followed by incubation in HRP conjugated anti-Rabbit (1:5,000, BS13278, Bioworld) or antimouse (1:5,000, BS12478, Bioworld) secondary antibody for 2 h at room temperature (RT). .. The signals were visualized using ECL substrate (180-5001, Tanon) on an automatic chemiluminescence/fluorescence image analysis system (Tanon 4600, Tanon).

    Article Title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse
    Article Snippet: .. Tissue powders or cells were extracted and homogenized with the lysis buffer containing 0.2% SDS (cells, lung, liver, brain, intestine, kidney) or 1% SDS (cochlea), 50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM ethylene diamine tetra-acetic acid (EDTA) pH 8.0, 1% Triton X-100 (A110694-0500, Sangon Biotech) and 1 mM phenylmethylsulfonyl fluoride (PMSF) (ST005, Beyotime) containing the cOmplete Protease Inhibitor Cocktail (11697498001, Roche) at 4°C for 10 min. Proteins were subjected to SDS–PAGE and transfer to PVDF membrane and analyzed by following antibodies: rabbit anti-Cingulin (1:1,000, 21369-1-AP, Proteintech), mouse anti-Cingulin (1:1,000, sc-365264, Santa Cruz), mouse anti-Flag (1:2,000, 30503ES60, Yeasen), rabbit anti-EGFP (1:2,000, 31002ES60, Yeasen), rabbit antiZO-1 (1:1,000, 21773-1-AP, Proteintech), rabbit anti-Occludin (1:1,000, 13409-1-AP, Proteintech), mouse anti-b-actin (1:20,000, 4970, Cell Signaling Technology) antibodies followed by incubation in HRP conjugated anti-Rabbit (1:5,000, BS13278, Bioworld) or antimouse (1:5,000, BS12478, Bioworld) secondary antibody for 2 h at room temperature (RT). .. The signals were visualized using ECL substrate (180-5001, Tanon) on an automatic chemiluminescence/fluorescence image analysis system (Tanon 4600, Tanon).

    Incubation:

    Article Title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse.
    Article Snippet: .. Tissue powders or cells were extracted and homogenized with the lysis buffer containing 0.2% SDS (cells, lung, liver, brain, intestine, kidney) or 1% SDS (cochlea), 50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM ethylene diamine tetra-acetic acid (EDTA) pH 8.0, 1% Triton X-100 (A110694-0500, Sangon Biotech) and 1 mM phenylmethylsulfonyl fluoride (PMSF) (ST005, Beyotime) containing the cOmplete Protease Inhibitor Cocktail (11697498001, Roche) at 4°C for 10 min. Proteins were subjected to SDS–PAGE and transfer to PVDF membrane and analyzed by following antibodies: rabbit anti-Cingulin (1:1,000, 21369-1-AP, Proteintech), mouse anti-Cingulin (1:1,000, sc-365264, Santa Cruz), mouse anti-Flag (1:2,000, 30503ES60, Yeasen), rabbit anti-EGFP (1:2,000, 31002ES60, Yeasen), rabbit antiZO-1 (1:1,000, 21773-1-AP, Proteintech), rabbit anti-Occludin (1:1,000, 13409-1-AP, Proteintech), mouse anti-b-actin (1:20,000, 4970, Cell Signaling Technology) antibodies followed by incubation in HRP conjugated anti-Rabbit (1:5,000, BS13278, Bioworld) or antimouse (1:5,000, BS12478, Bioworld) secondary antibody for 2 h at room temperature (RT). .. The signals were visualized using ECL substrate (180-5001, Tanon) on an automatic chemiluminescence/fluorescence image analysis system (Tanon 4600, Tanon).

    Article Title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse
    Article Snippet: .. Tissue powders or cells were extracted and homogenized with the lysis buffer containing 0.2% SDS (cells, lung, liver, brain, intestine, kidney) or 1% SDS (cochlea), 50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM ethylene diamine tetra-acetic acid (EDTA) pH 8.0, 1% Triton X-100 (A110694-0500, Sangon Biotech) and 1 mM phenylmethylsulfonyl fluoride (PMSF) (ST005, Beyotime) containing the cOmplete Protease Inhibitor Cocktail (11697498001, Roche) at 4°C for 10 min. Proteins were subjected to SDS–PAGE and transfer to PVDF membrane and analyzed by following antibodies: rabbit anti-Cingulin (1:1,000, 21369-1-AP, Proteintech), mouse anti-Cingulin (1:1,000, sc-365264, Santa Cruz), mouse anti-Flag (1:2,000, 30503ES60, Yeasen), rabbit anti-EGFP (1:2,000, 31002ES60, Yeasen), rabbit antiZO-1 (1:1,000, 21773-1-AP, Proteintech), rabbit anti-Occludin (1:1,000, 13409-1-AP, Proteintech), mouse anti-b-actin (1:20,000, 4970, Cell Signaling Technology) antibodies followed by incubation in HRP conjugated anti-Rabbit (1:5,000, BS13278, Bioworld) or antimouse (1:5,000, BS12478, Bioworld) secondary antibody for 2 h at room temperature (RT). .. The signals were visualized using ECL substrate (180-5001, Tanon) on an automatic chemiluminescence/fluorescence image analysis system (Tanon 4600, Tanon).



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